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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 15054-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAASCVLLH TGQKMPLIGL GTWKSEPGQV KAAVKYALSV GYRHIDCAAI YGNEPEIGEA LKEDVGPGKA VPREELFVTS KLWNTKHHPE DVEPALRKTL ADLQLEYLDL YLMHWPYAFE RGDNPFPKNA DGTICYDSTH YKETWKALEA LVAKGLVQAL GLSNFNSRQI DDILSVASVR PAVLQVECHP YLAQNELIAH CQARGLEVTA YSPLGSSDRA WRDPDEPVLL EEPVVLALAE KYGRSPAQIL LRWQVQRKVI CIPKSITPSR ILQNIKVFDF TFSPEEMKQL NALNKNWRYI VPMLTVDGKR VPRDAGHPLY PFNDPY (1-325 aa encoded by B C000670)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15054-1-AP (AKR1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-AKR1A1 (IP:15054-1-AP, 4ug; Detection:15054-1-AP 1:500) with HeLa cells lysate 3200ug.
