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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF4649 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Ryk Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and human Ryk in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor.
Povinelli BJ, Nemeth MJ
Stem cells (Dayton, Ohio) 2014 Jan;32(1):105-15
Stem cells (Dayton, Ohio) 2014 Jan;32(1):105-15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Ryk by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, NIH-3T3 mouse embryonic fibroblast cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Ryk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4649) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ryk at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Ryk in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse Ryk Affinity-purified Polyclonal Antibody (Catalog # AF4649, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
