LS-C775039

antibody from LSBio

Targeting: RYK D3S3195, JTK5, JTK5A, RYK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C775039

Provider

LSBio

Product name

RYK Antibody (HRP) LS-C775039

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Horseradish Peroxidase

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-RYK Polyclonal Antibody. Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.