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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- 22138-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RYK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: DDSISASSS SQGLSQPSTQ TTQYLRADTP NNATPITSSL GYPTLRIEKN DLRSVTLLEA KGKVKDIAIS RERITLKDVL QEGTFGRIFH GILIDEKDPN KEKQAFVKTV KDQASEIQVT MMLTESCKLR GLHHRNLLPI THVCIEEGEK PMVILPYMNW GNLKLFLRQC KLVEANNPQA ISQQDLVHMA IQIACGMSYL ARREVIHKDL AARNCVIDDT LQVKITDNAL SRDLFPMDYH CLGDNENRPV RWMALESLVN NEFSSASDVW AFGVTLWELM TLGQTPYVDI DPFEMAAYLK DGYRIAQPIN CPDELFAVMA CCWALDPEER PKFQQLVQCL TEFHAALGAY V (261-610 aa encoded by BC153090)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SH-SY5Y cells using 22138-1-AP (RYK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 40x lens).
