H00002827-M01

antibody from Abnova Corporation

Targeting: GPR3 ACCA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002827-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002827-M01, RRID:AB_425462

Product name

GPR3 monoclonal antibody (M01), clone 3B4-G3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GPR3.

Antigen sequence

MMWGAGSPLAWLSAGSGNVNVSSVGPAEGPTGPAA
PLPSPKAWDVVLCISGTLVSCENALVVAIIVGTPA
FRAPMFLLVGSLAVADLLAGLGLVLHFAAVFCIGS
AEMSLVLVGVLAMAFTASIGSLLAITVDRYLSLYN
ALTYYSETTVTRTYVMLALVWGGALGLGLLPVLAW
NCLDGLTTCGVVYPLSKNHLVVLAIAFFMVFGIML
QLYAQICRIVCRHAQQIALQRHLLPASHYVATRKG
IATLAVVLGAFAACWLPFTVYCLLGDAHSPPLYTY
LTLLPATYNSMINPIIYAFRNQDVQKVLWAVCCCC
SSSKIPFRSRSPSDV


Isotype

IgG

Antibody clone number

3B4-G3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GPR3 monoclonal antibody (M01), clone 3B4-G3 Western Blot analysis of GPR3 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GPR3 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol