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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00002827-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR3 Monoclonal Antibody (3B4-G3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MMWGAGSPLA WLSAGSGNVN VSSVGPAEGP TGPAAPLPSP KAWDVVLCIS GTLVSCENAL VVAIIVGTPA FRAPMFLLVG SLAVADLLAG LGLVLHFAAV FCIGSAEMSL VLVGVLAMAF TASIGSLLAI TVDRYLSLYN ALTYYSETTV TRTYVMLALV WGGALGLGLL PVLAWNCLDG LTTCGVVYPL SKNHLVVLAI AFFMVFGIML QLYAQICRIV CRHAQQIALQ RHLLPASHYV ATRKGIATLA VVLGAFAACW LPFTVYCLLG DAHSPPLYTY LTLLPATYNS MINPIIYAFR NQDVQKVLWA VCCCCSSSKI PFRSRSPSDV
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3B4-G3
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Cat # H00002827-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody (M01), clone 3B4-G3 (Cat # H00002827-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPR3 monoclonal antibody (M01), clone 3B4-G3 Western Blot analysis of GPR3 expression in Jurkat (Cat # L017V1).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GPR3 is approximately 0.03 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration is 1 µg/mL.
