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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB5422 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat EphB1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat EphB1 in direct ELISAs.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P09759- Isotype
- IgG
- Antibody clone number
- 917118
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- EphB1 in Rat Brain. EphB1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Mouse Anti-Rat EphB1 Monoclonal Antibody (Catalog # MAB5422) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in small-sized neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
