AP2785b

antibody from Abgent

Targeting: GADD45A DDIT1, GADD45

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2785b

Provider

Abgent

Proper citation

Abgent Cat#AP2785b, RRID:AB_10560375

Product name

GADD45A Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of GADD45A Antibody (C-term) (Cat. #AP2785b) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (C-term) (Cat. #AP2785b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

GADD45A Antibody (C-term) (Cat. #AP2785b) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50