655902

antibody from BioLegend

Targeting: WLS C1orf139, EVI, FLJ23091, GPR177, mig-14, MRP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

655902

Provider

BioLegend

Proper citation

BioLegend Cat#655902, RRID:AB_2562228

Product name

Purified anti-Wntless (GPR177)

Antibody type

Monoclonal

Antigen

Human Wntless protein

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

YJ5

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

4° C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Hela(lane1) and HT29(lane2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Wntless (clone YJ5) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection

Conjugate

Unconjugated