MA1-23273

antibody from Invitrogen Antibodies

Targeting: RBBP4 lin-53, NURF55, RbAp48

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

MA1-23273

Provider

Invitrogen Antibodies

Product name

RbAp48 Monoclonal Antibody (11G10)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

11G10

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.

Qian YW, Lee EY
The Journal of biological chemistry 1995 Oct 27;270(43):25507-13

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Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.

Qian YW, Lee EY
The Journal of biological chemistry 1995 Oct 27;270(43):25507-13

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RbAp48 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RbAp48 monoclonal antibody (Product # MA1-23273) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RbAp48 in HeLa whole cell and nuclear extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with RbAp48 Monoclonal antibody (Product # MA1-23273) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RbAp48 was performed by separating 30 µg of SH-SY5Y whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RbAp48 Monoclonal Antibody (11G10) (Product # MA1-23273) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RbAp48 Monoclonal Antibody (11G10) (Product # MA1-23273). HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 Monoclonal Antibody (11G10) (Product # MA1-23273) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of RbAp48 was achieved by transfecting HeLa with RbAp48 specific siRNAs (Silencer® select Products # s11838, s11837). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the RbAp48 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with RbAp48 Monoclonal Antibody (11G10) (Product # MA1-23273, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction in signal upon siRNA mediated knock down confirms that antibody is specific to RbAp48.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-RbAp48 Monoclonal Antibody (11G10) (Product # MA1-23273) and a 50 kDa band corresponding to RbAp48 was observed in all cell and tissue lysates tested. A band at ~ 25 kDa corresponding to circulating IgG was observed in mouse tissues. Modified whole cell lysates (1% SDS) (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), PANC-1 (Lane 4), tissue lysates (30 µg lysate) of Mouse Lung (Lane 5), Mouse Spleen (Lane 6), Rat Lung (Lane 7), Rat Stomach (Lane 8), and Rat Spleen (Lane 9) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RbAp48 in HeLa cells using a RbAp48 monoclonal antibody (Product # MA1-23273) at a 1:100 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa, using RbAp48 (Product # MA1-23273) antibody at 1:100 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis using RbAp48 Monoclonal Antibody (11G10) (Product # MA1-23273).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract. A: 5 µg preimmune mouse IgG. B: 5 µg of RbAp48 antibody (Product # MA1-23273). The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.