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LearnLS-C331493
antibody from LSBio
Targeting: RBBP4
lin-53, NURF55, RbAp48
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- LS-C331493 - Provider product page
- Provider
- LSBio
- Product name
- RBBP4 / RBAP48 Antibody LS-C331493
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using RBBP4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RBBP4 antibody. Western blot was performed from the immunoprecipitate using RBBP4 antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis extracts of 293T cells, using RBBP4 antibodyand rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RBBP4 antibody. Western blot was performed from the immunoprecipitate using RBBP4 antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RBBP4 antibody. Western blot was performed from the immunoprecipitate using RBBP4 antibodyat a dilition of 1:1000.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse lung using RBBP4 Antibodyat dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat liver using RBBP4 Antibodyat dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat kidney using RBBP4 Antibodyat dilution of 1:100 (40x lens).
