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LearnNBP1-41201
antibody from Novus Biologicals
Targeting: RBBP4
lin-53, NURF55, RbAp48
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP1-41201 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41201, RRID:AB_2238239
- Product name
- Mouse Monoclonal RBBP4/RbAp48 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody will recognize human RbAp48.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The depletion of PHF6 decreases the drug sensitivity of T-cell acute lymphoblastic leukemia to prednisolone.
Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin.
Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex.
Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.
Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD.
Xiang J, Wang G, Xia T, Chen Z
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Jan;109:2210-2217
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Jan;109:2210-2217
Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin.
Sun JM, Chen HY, Davie JR
The Journal of biological chemistry 2007 Nov 9;282(45):33227-36
The Journal of biological chemistry 2007 Nov 9;282(45):33227-36
Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex.
Skowyra D, Zeremski M, Neznanov N, Li M, Choi Y, Uesugi M, Hauser CA, Gu W, Gudkov AV, Qin J
The Journal of biological chemistry 2001 Mar 23;276(12):8734-9
The Journal of biological chemistry 2001 Mar 23;276(12):8734-9
Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.
Humphrey GW, Wang Y, Russanova VR, Hirai T, Qin J, Nakatani Y, Howard BH
The Journal of biological chemistry 2001 Mar 2;276(9):6817-24
The Journal of biological chemistry 2001 Mar 2;276(9):6817-24
Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD.
Schultz DC, Friedman JR, Rauscher FJ 3rd
Genes & development 2001 Feb 15;15(4):428-43
Genes & development 2001 Feb 15;15(4):428-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune mouse IgG, C. Immunoprecipitation of RbAp48 protein by 2. 5 ug of RbAp48 antibody 10% SDS-PAGE gel.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG, B. 5 ug of RbAp48 antibody The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
