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LearnNB500-123
antibody from Novus Biologicals
Targeting: RBBP4
lin-53, NURF55, RbAp48
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Blocking/Neutralizing Functional assay Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NB500-123 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-123, RRID:AB_10002950
- Product name
- Mouse Monoclonal RBBP4/RbAp48 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for full length RbAp48.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Structural and functional characterization of the RBBP4-ZNF827 interaction and its role in NuRD recruitment to telomeres.
Interaction of histone acetylases and deacetylases in vivo.
Interaction of histone acetylases and deacetylases in vivo.
Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex.
The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein.
The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein.
RbAp48 belongs to the histone deacetylase complex that associates with the retinoblastoma protein.
RbAp48 belongs to the histone deacetylase complex that associates with the retinoblastoma protein.
Yang SF, Sun AA, Shi Y, Li F, Pickett HA
The Biochemical journal 2018 Aug 31;475(16):2667-2679
The Biochemical journal 2018 Aug 31;475(16):2667-2679
Interaction of histone acetylases and deacetylases in vivo.
Yamagoe S, Kanno T, Kanno Y, Sasaki S, Siegel RM, Lenardo MJ, Humphrey G, Wang Y, Nakatani Y, Howard BH, Ozato K
Molecular and cellular biology 2003 Feb;23(3):1025-33
Molecular and cellular biology 2003 Feb;23(3):1025-33
Interaction of histone acetylases and deacetylases in vivo.
Yamagoe S, Kanno T, Kanno Y, Sasaki S, Siegel RM, Lenardo MJ, Humphrey G, Wang Y, Nakatani Y, Howard BH, Ozato K
Molecular and cellular biology 2003 Feb;23(3):1025-33
Molecular and cellular biology 2003 Feb;23(3):1025-33
Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex.
Zegerman P, Canas B, Pappin D, Kouzarides T
The Journal of biological chemistry 2002 Apr 5;277(14):11621-4
The Journal of biological chemistry 2002 Apr 5;277(14):11621-4
The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein.
Nicolas E, Ait-Si-Ali S, Trouche D
Nucleic acids research 2001 Aug 1;29(15):3131-6
Nucleic acids research 2001 Aug 1;29(15):3131-6
The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein.
Nicolas E, Ait-Si-Ali S, Trouche D
Nucleic acids research 2001 Aug 1;29(15):3131-6
Nucleic acids research 2001 Aug 1;29(15):3131-6
RbAp48 belongs to the histone deacetylase complex that associates with the retinoblastoma protein.
Nicolas E, Morales V, Magnaghi-Jaulin L, Harel-Bellan A, Richard-Foy H, Trouche D
The Journal of biological chemistry 2000 Mar 31;275(13):9797-804
The Journal of biological chemistry 2000 Mar 31;275(13):9797-804
RbAp48 belongs to the histone deacetylase complex that associates with the retinoblastoma protein.
Nicolas E, Morales V, Magnaghi-Jaulin L, Harel-Bellan A, Richard-Foy H, Trouche D
The Journal of biological chemistry 2000 Mar 31;275(13):9797-804
The Journal of biological chemistry 2000 Mar 31;275(13):9797-804
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody. The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
