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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- MAB2686 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PI 3-Kinase p110 beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human PI 3-kinase p110 beta in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P42338- Isotype
- IgG
- Antibody clone number
- 269020
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis.
Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y
Critical care medicine 2009 Oct;37(10):2791-9
Critical care medicine 2009 Oct;37(10):2791-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PI 3-Kinase p110 beta by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 beta Monoclonal Antibody (Catalog # MAB2686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 beta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
