H00011070-M01

antibody from Abnova Corporation

Targeting: TMEM115 PL6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011070-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011070-M01, RRID:AB_425880

Product name

PL6 monoclonal antibody (M01), clone 3D4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PL6.

Antigen sequence

MQRALPGARQHLGAILASASVVVKALCAAVLFLYL
LSFAVDTGCLAVTPGYLFPPNFWIWTLATHGLMEQ
HVWDVAISLTTVVVAGRLLEPLWGALELLIFFSVV
NVSVGLLGAFAYLLTYMASFNLVYLFTVRIHGALG
FLGGVLVALKQTMGDCVVLRVPQVRVSVMPMLLLA
LLLLLRLATLLQSPALASYGFGLLSSWVYLRFYQR
HSRGRGDMADHFAFATFFPEILQPVVGLLANLVHS
LLVKVKICQKTVKRYDVGAPSSITISLPGTDPQDA
ERRRQLALKALNERLKRVEDQSIWPSMDDDEEESG
AKVDSPLPSDKAPTPPGKGAAPESSLITFEAAPPT
L


Isotype

IgG

Antibody clone number

3D4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport.

Ong YS, Tran TH, Gounko NV, Hong W
Journal of cell science 2014 Jul 1;127(Pt 13):2825-39

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PL6 monoclonal antibody (M01), clone 3D4 Western Blot analysis of PL6 expression in HepG2 ( Cat # L019V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TMEM115 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol