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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
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- Product number
- PA5-96557 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COPB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AYQLAVEAES EQKWKQLAEL AISKCQFGLA QECLHHAQDY GGLLLLATAS GNANMVNKLA EGAERDGKNN VAFMSYFLQG KVDACLELLI RTGRLPEAAF LARTYLPSQV SRVVKLWREN LSKVNQKAAE SLADPTEYEN LFPGLKEAFV VEEWVKETHA DLWPAKQYPL VTPNEERNVM EEGKDFQPSR STAQQELDGK PASPTPVIVA SHTANKEEKS LLELEVDLDN LELEDIDTTD INLDEDILDD; Positive Samples: SW480, MCF7, HeLa, HepG2, Jurkat, Mouse liver, Mouse kidney, Mouse stomach; Cellular Location: COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.54 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COPB2 in extracts of various cell lines using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using COPB2 Polyclonal Antibody (Product # PA5-96557) diluted 1:1,000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) diluted 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL. Exposure time: 180 s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COPB2 was performed in HeLa cells using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COPB2 was performed in NIH/3T3 cells using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of COPB2 was performed in PC-12 cells using COPB2 Polyclonal Antibody (Product # PA5-96557).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPB2 in paraffin-embedded rat brain using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPB2 in paraffin-embedded rat heart using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPB2 in paraffin-embedded mouse brain using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPB2 in paraffin-embedded mouse kidney using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COPB2 in paraffin-embedded mouse heart using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:200.
