- MA5-15730 - Invitrogen Antibodies SLC22A1 antibody

Yang H, Zhou S, Guo D, Obianom ON, Li Q, Shu Y
Pharmaceutics 2021 Apr 13;13(4)

Chow JPH, Cai Y, Chow DTL, Chung SHK, Chau KC, Ng KY, Leung OM, Wong RMH, Law AWL, Leung YO, Kwok SY, Leung YC
PloS one 2020;15(4):e0231633

Obianom ON, Coutinho AL, Yang W, Yang H, Xue F, Shu Y
Molecular pharmaceutics 2017 Aug 7;14(8):2726-2739

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SLC22A1 using SLC22A1 monoclonal antibody (Product # MA5-15730) in HEK293 (1) and SLC22A1 (AA: 284-347) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SLC22A1 using SLC22A1 monoclonal antibody (Product # MA5-15730) in HEK293 (1) and SLC22A1 (AA: 284-347) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of Jurkat cells using SLC22A1 monoclonal antibody (Product # MA5-15730) (green) and negative control (purple).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 4 Expression of ASS1 in various cell lines and the effect of NEI-01 on their cell viability. (A) Expression of ASS1, ASL and OTC in SMMC7721, MIA PaCa-2 and PANC-1. Unperturbed asynchronously growing cells were harvested by trypsinization. After cell lysis, total cell lysates were subject to immunoblotting with anti-ASS1, anti-ASL, anti-OTC or anti-ACTIN antibodies. (B) Inhibition of cell growth of MIA PaCa-2 and PANC-1 cells but not SMMC7721 cells by NEI-01 treatment. SMMC7721 cells, MIA PaCa-2 cells or PANC-1 were seeded in 96 well-plate with ~3000 cells/well density 24 hr prior to NEI-01 treatments. After 72 hr, cells were subject to MTT assay. The relative cell viability in % vs the NEI-01 concentration was shown as mean +- SD of triplicate wells. (C) Activation of autophagic pathway upon NEI-01 treatment. MIA PaCa-2 cells were treated with indicated amount of NEI-01 for 3 or 7 days. The cells were then harvested and subject to immunoblotting using designated antibodies.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 6 Expression of ASS1 upon long-term NEI-01 treatment. ( A) Re-expression of ASS1 in NEI-01 treated MIA-PaCa-2 cells. MIA-PaCa-2 cells were propagated in DMEM supplemented with 0.01 mug/ml of NEI-01. Cells were harvested at indicated time points and subject to immunoblotting using anti-ASS1 and anti-ACTIN antibodies. Unperturbed asynchronously growing SMMC7721 cells were used as a control. (B) Reduction of ASS1 expression in NEI-01-adapted MIA PaCa-2 cells after removing of NEI-01 in the medium. MIA PaCa-2 cells adapted to NEI-01 from (A) were washed with PBS and grown in DMEM without NEI-01. The cells were harvested at indicated time points and subject to immunoblotting using anti-ASS1 and anti-ACTIN antibodies. (C) Resensitizing NEI-01-adapted cells to NEI-01. NEI-01-adapted MIA PaCa-2 cells from (A) and grown in medium without NEI-01 for 96 hr from (B) were seeded in 96 well-plate with ~3000 cells/well density 24 hr prior to NEI-01 treatments. After 72 hr, cells were subject to MTT assay. The relative cell viability in % vs the NEI-01 concentration was shown as mean +- SD of triplicate wells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Cd 2+ exposure increases membrane expression of hOCT1 and hOCT2 but not hMATE1. ( a ) Immunoblotting (IB) analysis of total and membrane fraction of hOCT2 in HEK-hOCT2 cells with and without exposure to different concentrations of Cd 2+ for 20 min. ( b ) IB analysis of total and membrane fraction of hOCT1 in HEK-hOCT1 cells with and without exposure to Cd 2+ for 20 min. ( c ) IB analysis of total and membrane fraction of hMATE1 in HEK-hMATE1 cells with and without exposure to different concentrations of Cd 2+ for 20 min. All figures are representatives of three independent experiments. Pan-cadherin and Na + /K + ATPase are served as the positive control and beta-actin as negative control for membrane proteins.

MA5-15730