- PA5-62768 - Invitrogen Antibodies AGPAT4 antibody

Submitted references The Agpat4/LPA axis in colorectal cancer cells regulates antitumor responses via p38/p65 signaling in macrophages.

Zhang D, Shi R, Xiang W, Kang X, Tang B, Li C, Gao L, Zhang X, Zhang L, Dai R, Miao H
Signal transduction and targeted therapy 2020 Mar 27;5(1):24

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent staining of AGPAT4 in human cell line U-251 MG shows positivity in vesicles. Samples were probed using an AGPAT4 Polyclonal Antibody (Product # PA5-62768).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical staining of AGPAT4 in human testis tissue shows strong cytoplasmic positivity in cells in seminiferus ducts. Samples were probed using an AGPAT4 Polyclonal Antibody (Product # PA5-62768).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 1 Aberrant upregulation of Agpat4 in colorectal cancer cells. a - c Paracarcinoma (Pc) and carcinoma (Ca) tissues were collected from patients with colorectal cancer (CRC) and subjected to RNA-sequencing analysis. Each tested sample was pooled from three individual samples. KEGG ( a ) or GO ( b, c ) pathway enrichment was performed as indicated. The pathways marked by red boxes were focused on in subsequent analyses. b , c The vertical axis indicates the GO term, and the horizontal axis indicates the Rich factor, which is defined as the ratio of enriched gene number to background gene number. The size of the circles indicates the gene number in the GO term, and the color of the circles indicates the false discovery rate (FDR)-corrected P value. d Heat map showing the relative expression of acylglycerol biosynthetic process-related genes in Pc and Ca tissues from CRC patients. e Concentrations of LPA in Pc and Ca tissues from CRC patients. ( n = 6). f - j Relative mRNA levels of AGPAT1, AGPAT2, AGPAT3, AGPAT4, and AGPAT5 in the Pc and Ca tissues from CRC patients. k Representative images showing the immunostaining of AGPAT4 in Pa and Ca tissues from CRC patients. The statistical data were obtained by analyzing 15 pairs of samples from 15 patients. Scale bar represents 200 mum. ( n = 15). The data in ( e - k ) represent the means +- s.e.ms. (** P < 0.01, *** P < 0.005; Student's t test)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2. Agpat4 silencing in CRC inhibits tumor progression. a Immunoblotting assays of Agpat4 in MC-38 cells transfected with Agpat4 shRNAs (sh-A1, sh-A2, and sh-A3) or the PLKO vector as a control (sh-NC). b Statistical data showing the gray values in a. (n= 3). c Real-time PCR assays of Agpat4 in MC-38 cells transfected with sh-NC, sh-A2, or sh-A3. (n= 6). d Six-week-old male C57BL/6 mice were intraperitoneally inoculated with sh-A2- or sh-NC-transfected MC-38 cells (410^6 cells in 100 µl of PBS for each mouse). Two weeks later, mice were sacrificed, and the tumor nodules were collected and displayed. (n= 4-5). e Six-week-old male C57BL/6 mice were subcutaneously injected with sh-A2- or sh-NC-transfected MC-38 cells (4 10^6 cells in 100 µl of PBS for each mouse) for 15 days. Then, the mice were sacrificed, and the tumors were collected. (n= 5). f Six-week-old male C57BL/6 mice were intraperitoneally inoculated with sh-A3- or sh-NC-transfected MC-38 cells (4 10^6 cells in 100 µl of PBS for each mouse). Two weeks later, the mice were sacrificed, and the tumor nodules were collected and displayed. (n= 4). g Six-week-old male C57BL/6 mice were subcutaneously engrafted with sh-A3- or sh-NC-transfected MC-38 cells (4 10^6 cells in 100 µl of PBS for each mouse) for 15 days. Then, the mice were sacrificed, and the tumors were collected. (n= 3). h Immunoblotting assays of Agpat4 in CT-26 cells transfected with sh-A2 or sh-NC. sh-A2 was used for Agpat4 silencing (sh-Agpa

PA5-62768

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