H00011080-M02

antibody from Abnova Corporation

Targeting: DNAJB4 HLJ1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011080-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011080-M02, RRID:AB_10720058

Product name

DNAJB4 monoclonal antibody (M02), clone S13

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant DNAJB4.

Antigen sequence

MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDK
NKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEE
GLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGS
NPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMN
GYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGC
TKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKE
GTKITFPREGDETPNSIPADIVFIIKDKDHPKFKR
DGSNIIYTAKISLREALCGCSINVPTLDGRNIPMS
VNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFE
VSFPDTISSSSKEVLRKHLPAS


Isotype

IgG

Antibody clone number

S13

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DNAJB4 expression in transfected 293T cell line by DNAJB4 monoclonal antibody (M02), clone S13.Lane 1: DNAJB4 transfected lysate(37.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DNAJB4 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of DNAJB4 transfected lysate using anti-DNAJB4 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DNAJB4 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol