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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 67966-1-IG150UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJB4 Monoclonal Antibody (1F3H7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F3H7
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates (A549, HeLa, HEK-293, Jurkat, K-562, pig heart, rabbit heart) were subjected to SDS PAGE followed by western blot with (Product # 67966-1-IG150UL) (DNAJB4 antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with (Product # 67966-1-IG150UL) (DNAJB4 antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody ((Product # 67966-1-IG150UL), Clone: 1F3H7 ) at dilution of 1:800 and CoraLite 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using (Product # 67966-1-IG150UL) (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using (Product # 67966-1-IG150UL) (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
