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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB6472 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6472, RRID:AB_10679632
- Product name
- PRNP monoclonal antibody, clone EM-20
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant PRNP.
- Isotype
- IgG
- Antibody clone number
- EM-20
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references Binding of pro-prion to filamin A disrupts cytoskeleton and correlates with poor prognosis in pancreatic cancer.
Glycosylation modification of human prion protein provokes apoptosis in HeLa cells in vitro.
The cellular prion protein interacts with the tissue non-specific alkaline phosphatase in membrane microdomains of bioaminergic neuronal cells.
Li C, Yu S, Nakamura F, Yin S, Xu J, Petrolla AA, Singh N, Tartakoff A, Abbott DW, Xin W, Sy MS
The Journal of clinical investigation 2009 Sep;119(9):2725-36
The Journal of clinical investigation 2009 Sep;119(9):2725-36
Glycosylation modification of human prion protein provokes apoptosis in HeLa cells in vitro.
Yang Y, Chen L, Pan HZ, Kou Y, Xu CM
BMB reports 2009 Jun 30;42(6):331-7
BMB reports 2009 Jun 30;42(6):331-7
The cellular prion protein interacts with the tissue non-specific alkaline phosphatase in membrane microdomains of bioaminergic neuronal cells.
Ermonval M, Baudry A, Baychelier F, Pradines E, Pietri M, Oda K, Schneider B, Mouillet-Richard S, Launay JM, Kellermann O
PloS one 2009 Aug 4;4(8):e6497
PloS one 2009 Aug 4;4(8):e6497
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- Experimental details
- Western Blotting analysis of Creutzfeld-Jakob disease (CJD) negative (Lane 1, 2) and CJD positive (Lane 3, 4) human brain material using PRNP monoclonal antibody, clone EM-20 (Cat # MAB6472). CJD positive patient has proteinase K resistent prion protein.Lane 1, 4 : Samples with proteinase K treatment Lane 2, 3 : Samples without proteinase K treatment.
