Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 12-9230-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD230 (PrP) Monoclonal Antibody (4D5), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: CD230 (prion protein; PrP^C) is a 35 kD glycoprotein, attached to the cell surface via a GPI-anchor. While the function of CD230 is unclear, prion protein has been the focus of research based on its involvement in transmissible spongiform encephalopathies (TSE), including Creutzfeld-Jakob disease in humans. CD230 undergoes a conformational change in which the cellular PrP^C form is converted to the scrapie PrP^Sc form. This conversion is essential for the infectiousness of prion-based diseases. CD230 is predominantly expressed on the surface of lymphocytes and monocytes, with weak expression on granulocytes and erythrocytes. In platelets, CD230 is expressed primarily intracellularly, and is upregulated to the surface upon activation. Applications Reported: This 4D5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4D5 antibody has been pre-titrated and tested. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 4D5
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins.
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Novel flow cytometric analysis of the blood-brain barrier.
Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.
Activated platelets of patients with paroxysmal nocturnal hemoglobinuria express cellular prion protein.
Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes.
Distribution of cell-associated prion protein in normal adult blood determined by flow cytometry.
Liu SS, Liu YS, Guo XY, Murakami Y, Yang G, Gao XD, Kinoshita T, Fujita M
Communications biology 2021 Jun 23;4(1):777
Communications biology 2021 Jun 23;4(1):777
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Novel flow cytometric analysis of the blood-brain barrier.
Williams DW, Tesfa L, Berman JW
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Oct;87(10):897-907
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Oct;87(10):897-907
Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.
Davis EM, Kim J, Menasche BL, Sheppard J, Liu X, Tan AC, Shen J
Cell reports 2015 Jun 23;11(11):1727-36
Cell reports 2015 Jun 23;11(11):1727-36
Activated platelets of patients with paroxysmal nocturnal hemoglobinuria express cellular prion protein.
Holada K, Simak J, Risitano AM, Maciejewski J, Young NS, Vostal JG
Blood 2002 Jul 1;100(1):341-3
Blood 2002 Jul 1;100(1):341-3
Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes.
Dürig J, Giese A, Schulz-Schaeffer W, Rosenthal C, Schmücker U, Bieschke J, Dührsen U, Kretzschmar HA
British journal of haematology 2000 Mar;108(3):488-95
British journal of haematology 2000 Mar;108(3):488-95
Distribution of cell-associated prion protein in normal adult blood determined by flow cytometry.
Barclay GR, Hope J, Birkett CR, Turner ML
British journal of haematology 1999 Dec;107(4):804-14
British journal of haematology 1999 Dec;107(4):804-14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human CD230 (PrP) PE (filled histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye