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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 14446-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RSK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GQGSYGKVF LVRKVKGSDA GQLYAMKVLK KATLKVRDRV RSKMERDILA EVNHPFIVKL HYAFQTEGKL YLILDFLRGG DLFTRLSKEV MFTEEDVKFY LAELALALDH LHSLGIIYRD LKPENILLDE EGHIKITDFG LSKEAIDHDK RAYSFCGTIE YMAPEVVNRR GHTQSADWWS FGVLMFEMLT GSLPFQGKDR KETMALILKA KLGMPQFLSG EAQSLLRALF KRNPCNRLGA GIDGVEE (66-311 aa encoded by BC002363)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14446-1-AP (RSK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RSK3 (IP:14446-1-AP, 4ug; Detection:14446-1-AP 1:800) with HeLa cells lysate 4000ug.
