MA5-38625

antibody from Invitrogen Antibodies

Targeting: RPS6KA2 HU-2, RSK3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38625

Provider

Invitrogen Antibodies

Product name

RSK3 Monoclonal Antibody (3C4C8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody has been tested in indirect ELISA.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3C4C8

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HeLa (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HEK293 (1) and RPS6KA2 (AA: 415-734)-human IgG-Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HeLa (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using RSK3 Monoclonal Antibody (Product # MA5-38625) against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HEK293 (1) and RPS6KA2 (AA: 415-734)-human IgG-Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of RSK3 in A431 cells using RSK3 Monoclonal Antibody (Product # MA5-38625) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RSK3 in paraffin-embedded cervical cancer tissues using RSK3 Monoclonal Antibody (Product # MA5-38625) and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RSK3 in paraffin-embedded lung cancer tissues using RSK3 Monoclonal Antibody (Product # MA5-38625) and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of RSK3 in HeLa cells using RSK3 Monoclonal Antibody (Product # MA5-38625) (green) and negative control (red).