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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab126068 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant RSK3 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human RSK3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500 - 1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200 Protein FunctionSerine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.Post-translationalActivated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1. N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 343-362- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant RSK3 Antibody (Ab126068) - Western Blot All lanes: Recombinant RSK3 Antibody (Ab126068) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 83 kDa Observed band size: 84 kDa Exposure time: 11.1 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant RSK3 Antibody (Ab126068) - IF/ICC Immunocytochemistry analysis of RSK3 (green) in U87-MG using Recombinant RSK3 Antibody (Ab126068), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant RSK3 Antibody (Ab126068) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant RSK3 Antibody (Ab126068). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
