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LearnH00006196-M01
antibody from Novus Biologicals
Targeting: RPS6KA2
HU-2, RSK3
Western blot
ELISA Immunocytochemistry Immunohistochemistry Blocking/Neutralizing Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00006196-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006196-M01, RRID:AB_2181451
- Product name
- Mouse Monoclonal RSK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RPS6KA2 (1F6)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references p90 ribosomal S6 kinase 3 contributes to cardiac insufficiency in α-tropomyosin Glu180Gly transgenic mice.
Passariello CL, Gayanilo M, Kritzer MD, Thakur H, Cozacov Z, Rusconi F, Wieczorek D, Sanders M, Li J, Kapiloff MS
American journal of physiology. Heart and circulatory physiology 2013 Oct 1;305(7):H1010-9
American journal of physiology. Heart and circulatory physiology 2013 Oct 1;305(7):H1010-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi ( Cat # H00006196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody (M01) clone 1F6 (Cat # H00006196-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody (M01), clone 1F6.Lane 1: RPS6KA2 transfected lysate(83.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - RPS6KA2 monoclonal antibody (M01), clone 1F6 Analysis of RPS6KA2 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of monoclonal antibody to RPS6KA2 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.
