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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-26004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospholamban Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inorganic arsenic exposure induces sex-disparate effects and exacerbates ischemia-reperfusion injury in the female heart.
Veenema R, Casin KM, Sinha P, Kabir R, Mackowski N, Taube N, Bedja D, Chen R, Rule A, Kohr MJ
American journal of physiology. Heart and circulatory physiology 2019 May 1;316(5):H1053-H1064
American journal of physiology. Heart and circulatory physiology 2019 May 1;316(5):H1053-H1064
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2) and Mouse Kidney (Lane 3). The blot was probed with Anti-Phospholamban Polyclonal Antibody (Product # PA5-26004, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 6 kDa band corresponding to Phospholamban was observed in Mouse and Rat Heart tissue when compared to Mouse Kidney.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2) and Mouse Kidney (Lane 3). The blot was probed with Anti-Phospholamban Polyclonal Antibody (Product # PA5-26004, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 6 kDa band corresponding to Phospholamban was observed in Mouse and Rat Heart tissue when compared to Mouse Kidney.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospholaman in formalin-fixed, paraffin-embedded mouse heart tissue using a Phospholamban polyclonal antibody (Product # PA5-26004), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells using a Phospholamban polyclonal antibody (Product # PA5-26004) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody.
