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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA5-24325 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MGAT5 Monoclonal Antibody (706824)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 706824
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of mouse Anti-human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Product # MA5-24325) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MGAT5 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in MGAT5 monoclonal antibody (Product # MA5-24325) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for N‚Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MGAT5 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in MGAT5 monoclonal antibody (Product # MA5-24325) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MGAT5 in immersion fixed MCF‚7 human breast cancer cell line. Samples were incubated in MGAT5 monoclonal antibody (Product # MA5-24325) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using mouse Anti-human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Product # MA5-24325) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm.
