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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21868 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HPS3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
- Concentration
- 0.92 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HPS3 Polyclonal Antibody (Product # PA5-21868). Sample (30 µg of whole cell lysate). Lane A: 293T. B: A431. C: H1299. D: HeLa S3. E: Hep G2. F: MOLT4. G: Raji. 7.5% SDS PAGE. HPS3 Polyclonal Antibody (Product # PA5-21868) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HPS3 Polyclonal Antibody detects HPS3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HPS3 protein stained by HPS3 Polyclonal Antibody (Product # PA5-21868) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HPS3 (Product # PA5-21868) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
