11233-1-AP

antibody from Proteintech Group

Targeting: EIF2A EIF-2A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11233-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11233-1-AP, RRID:AB_2246321

Product name

EIF2A/CDA02 antibody

Antibody type

Polyclonal

Description

KD/KO validated EIF2A/CDA02 antibody (Cat. #11233-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Autophagy 2021 Nov;17(11):3592-3606

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The alternative initiation factor eIF2A plays key role in RAN translation of myotonic dystrophy type 2 CCUG•CAGG repeats.

Tusi SK, Nguyen L, Thangaraju K, Li J, Cleary JD, Zu T, Ranum LPW
Human molecular genetics 2021 May 31;30(11):1020-1029

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Local translation in nuclear condensate amyloid bodies.

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Journal of cellular and molecular medicine 2020 Nov;24(21):12411-12420

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Hydrogen sulfide regulates muscle RING finger-1 protein S-sulfhydration at Cys(44) to prevent cardiac structural damage in diabetic cardiomyopathy.

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British journal of pharmacology 2020 Feb;177(4):836-856

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Betulinic Acid Suppresses Breast Cancer Metastasis by Targeting GRP78-Mediated Glycolysis and ER Stress Apoptotic Pathway.

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Evaluation of the Antiviral Activity of Sephin1 Treatment and Its Consequences on eIF2α Phosphorylation in Response to Viral Infections.

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Correction to: Protective effects of dioscin against alcohol‑induced liver injury.

Xu T, Zheng L, Xu L, Yin L, Qi Y, Xu Y, Han X, Peng J
Archives of toxicology 2019 Sep;93(9):2713

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EIF2A promotes cell survival during paclitaxel treatment in vitro and in vivo.

Chen L, He J, Zhou J, Xiao Z, Ding N, Duan Y, Li W, Sun LQ
Journal of cellular and molecular medicine 2019 Sep;23(9):6060-6071

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Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.

Qin W, Wu X, Jia Y, Tong X, Guo C, Chen D, Wang Z, Tan N
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109188

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Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress.

Li F, Zhang Y, Lu X, Shi J, Gong Q
Life sciences 2019 Oct 1;234:116739

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Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

Zhou MF, Feng ZP, Ou YC, Peng JJ, Li K, Gong HD, Qiu BH, Liu YW, Wang YJ, Qi ST
CNS neuroscience & therapeutics 2019 May;25(5):562-574

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Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2.

Trulley P, Snieckute G, Bekker-Jensen D, Menon MB, Freund R, Kotlyarov A, Olsen JV, Diaz-Muñoz MD, Turner M, Bekker-Jensen S, Gaestel M, Tiedje C
Cell reports 2019 Jun 4;27(10):2859-2870.e6

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KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.

Li SS, Jiang WL, Xiao WQ, Li K, Zhang YF, Guo XY, Dai YQ, Zhao QY, Jiang MJ, Lu ZJ, Wan R
World journal of gastrointestinal oncology 2019 Aug 15;11(8):599-621

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Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Maryam A, Mehmood T, Yan Q, Li Y, Khan M, Ma T
Oxidative medicine and cellular longevity 2018;2018:3853409

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GanDouLing combined with Penicillamine improves cerebrovascular injury via PERK/eIF2α/CHOP endoplasmic reticulum stress pathway in the mouse model of Wilson's disease.

Chen Y, Zhang B, Cao S, Huang W, Liu N, Yang W
Bioscience reports 2018 Oct 31;38(5)

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ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.

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Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.

Ho JJD, Balukoff NC, Cervantes G, Malcolm PD, Krieger JR, Lee S
Cell reports 2018 Jan 2;22(1):17-26

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eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.

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Cellular and molecular life sciences : CMLS 2018 Dec;75(23):4287-4300

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Montelukast inhibits hypoxia inducible factor-1α translation in prostate cancer cells.

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Cancer biology & therapy 2018 Aug 3;19(8):715-721

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Nuclear factor E2-related factor 2 knockdown enhances glucose uptake and alters glucose metabolism in AML12 hepatocytes.

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Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress.

Maryam A, Mehmood T, Zhang H, Li Y, Khan M, Ma T
Scientific reports 2017 Jul 24;7(1):6242

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Endoplasmic reticulum stress and apoptosis via PERK-eIF2α-CHOP signaling in the methamphetamine-induced chronic pulmonary injury.

Gu YH, Wang Y, Bai Y, Liu M, Wang HL
Environmental toxicology and pharmacology 2017 Jan;49:194-201

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Translation from unconventional 5' start sites drives tumour initiation.

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eLife 2016 Dec 23;5

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Protective effects of dioscin against alcohol-induced liver injury.

Xu T, Zheng L, Xu L, Yin L, Qi Y, Xu Y, Han X, Peng J
Archives of toxicology 2014 Mar;88(3):739-53

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Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice.

Dong D, Zhang S, Yin L, Tang X, Xu Y, Han X, Qi Y, Peng J
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Science (New York, N.Y.) 2012 Jun 29;336(6089):1719-23

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eIF2A mediates translation of hepatitis C viral mRNA under stress conditions.

Kim JH, Park SM, Park JH, Keum SJ, Jang SK
The EMBO journal 2011 May 10;30(12):2454-64

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The antidepressant sertraline inhibits translation initiation by curtailing mammalian target of rapamycin signaling.

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Cancer research 2010 Apr 15;70(8):3199-208

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using EIF2A antibody 11233-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-EIF2A (IP:11233-1-AP, 3ug; Detection:11233-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 11233-1-AP(EIF2A antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue