Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 690302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#690302, RRID:AB_2629818
- Product name
- Purified anti-eIF-2A
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of human eIF-2A (448-576 a.a.) expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- P83A7A8
- Vial size
- 100 µg
- Concentration
- 0.5 μ/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell or tissue lysates (15 µg protein) from SH-SY5Y, HepG2, OVCAR3 and mouse brain were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-eIF-2A antibody (clone P83A7A8). Purified anti-GAPDH antibody (clone Poly6314) was used as a loading control. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated