MA5-31725

antibody from Invitrogen Antibodies

Targeting: EIF2A EIF-2A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31725

Provider

Invitrogen Antibodies

Product name

EIF2A Monoclonal Antibody (3A7A8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-31725 has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3A7A8

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of eIF2a in MCF-7 (1), PC-12 (2), HepG2 (3), HeLa (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate. Samples were incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of eIF2a in human EIF2A recombinant protein. Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of eIF2a in HEK293 (1) and eIF2a cell lysate. Samples were incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:500-1:2000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of eIF2a in HepG2 cells (green). Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eIF2a in paraffin-embedded cervical cancer tissue. Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of eIF2a in HepG2 cells (green). Sample was incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:200-1:400 followed by negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of eIF2a in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with eIF2a monoclonal antibody (Product # MA5-31725) using a dilution of 1:10,000.