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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324369 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal LMO4 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal LMO4 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- LMO4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
Submitted references LMO4 mediates trastuzumab resistance in HER2 positive breast cancer cells.
Ding K, Wu Z, Li X, Sheng Y, Wang X, Tan S
American journal of cancer research 2018;8(4):594-609
American journal of cancer research 2018;8(4):594-609
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LMO4 Antibody (Center) (Cat. #TA324369) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the LMO4 antibody detected the LMO4 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A549 cell stained with LMO4 Antibody (Center)(Cat#TA324369).A549 cells were incubated with LMO4 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).LMO4 immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LMO4 Antibody (Center) (Cat. #TA324369) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
