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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C482374 - Provider product page
- Provider
- LSBio
- Product name
- Anti-HMT / HNMT Antibody LS-C482374
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 50µl
- Storage
- Aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of HNMT expression in HepG2 (A); HeLa (B); mouse liver (C); mouse brain (D) whole cell lysates.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HNMT staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
