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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-109825 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HNMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MASSMRSLFS DHGKYVESFR RFLNHSTEHQ CMQEFMDKKL PGIIGRIGDT KSEIKILSIG GGAGEIDLQI LSKVQAQYPG VCINNEVVEP SAEQIAKYKE LVAKTSNLEN VKFAWHKETS SEYQSRMLEK KELQKWDFIH MIQMLYYVKD IPATLKFFHS LLGTNAKMLI IVVSGSSGWD KLWKKYGSRF PQDDLCQYIT SDDLTQMLDN LGLKYECYDL LSTMDISDCF IDGNENGDLL WDFLTETCNF NATAPPDLRA ELGKDLQEPE FSAKKEGKVL FNNTLSFIVI EA
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.41 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HNMT in 22RV1, NCI-H460, HT-29, HepG2, HeLa, mouse liver, mouse brain. Samples were incubated in polyclonal HNMT antibody (Product # PA5-109825) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HNMT in 22RV1, NCI-H460, HT-29, HepG2, HeLa, mouse liver, mouse brain. Samples were incubated in polyclonal HNMT antibody (Product # PA5-109825) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HNMT in U-2OS cells. Samples were incubated in polyclonal HNMT antibody (Product # PA5-109825) using a dilution of 1:100, followed by DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of HNMT in HT-29 cells. Samples were incubated in polyclonal HNMT antibody (Product # PA5-109825) using a dilution of 1:1000.
