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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP11417PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11417PU-N, RRID:AB_1753836
- Product name
- anti HNMT / HMT (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the C-terminal region of human HNMT
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HNMT (arrow) using rabbit polyclonal HNMT Antibody (C-term). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the HNMT gene (Lane 2) (Origene Technologies).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-HNMT Antibody (C-term) (AP11417PU-N) in HepG2 cell line lysates (35 µg/lane). HNMT (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT Antibody (C-term) (AP11417PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
