Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF7637 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Histamine N-Methyltransferase/HNMT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Histamine N-Methyltransferase/HNMT in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P50135
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Histamine N-Methyltransferase/HNMT by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, human stomach tissue, human kidney tissue, and human lung tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Histamine N-Methyltransferase/HNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histamine N-Methyltransferase/HNMT at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.