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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28197 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OTC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HepG2, mouse Liver. Predicted reactivity: Mouse (94%), Rat (93%), Pig (94%), Chicken (80%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.39 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Targeted mRNA Therapy for Ornithine Transcarbamylase Deficiency.
Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.
Prieve MG, Harvie P, Monahan SD, Roy D, Li AG, Blevins TL, Paschal AE, Waldheim M, Bell EC, Galperin A, Ella-Menye JR, Houston ME
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Mar 7;26(3):801-813
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Mar 7;26(3):801-813
Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.
Meierhofer D, Halbach M, Şen NE, Gispert S, Auburger G
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
Molecular & cellular proteomics : MCP 2016 May;15(5):1728-39
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using OTC Polyclonal Antibody (Product # PA5-28197). Sample (30 µg of whole cell lysate). A: A431. 12% SDS PAGE. OTC Polyclonal Antibody (Product # PA5-28197) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using OTC Polyclonal Antibody (Product # PA5-28197). Sample (20 µg of whole cell lysate). Lane A: mouse Liver. 10% SDS PAGE. OTC Polyclonal Antibody (Product # PA5-28197) diluted at 1:30,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC (Product # PA5-28197) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
