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- Antibody Data
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- Product number
- PA5-31530 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGAP15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat.
- Concentration
- 1 mg/mL
Submitted references Decreased expression of ARHGAP15 promotes the development of colorectal cancer through PTEN/AKT/FOXO1 axis.
Pan S, Deng Y, Fu J, Zhang Y, Zhang Z, Ru X, Qin X
Cell death & disease 2018 Jun 4;9(6):673
Cell death & disease 2018 Jun 4;9(6):673
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ARHGAP15 Polyclonal Antibody (Product # PA5-31530). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 10% SDS PAGE. ARHGAP15 Polyclonal Antibody (Product # PA5-31530) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ARHGAP15 in methanol-fixed HeLa cells using an ARHGAP15 polyclonal antibody (Product # PA5-31530) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft, using ARHGAP15 (Product # PA5-31530) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Downregulation of ARHGAP15 in CRC tissues. a , b ARHGAP15 mRNA expression was analyzed based on two public available data sets: GSE9348 ( a ) and TCGA CRC ( b ). c The mRNA levels of ARHGAP15 on our own cohort were determined using qRT-PCR. d The protein levels of ARHGAP15 were determined by immunohistochemistry staining. Scale bar: 100 mum. e Kaplan-Meier survival curves showing the difference of survival time between patients with low and high expression of ARHGAP15 on CRC samples from 95 patients. **** P < 0.0001
