Antibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 13220-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13220-1-AP, RRID:AB_2109348
- Product name
- GBP5 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GBP5 antibody (Cat. #13220-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Macrophagic HDAC3 inhibition ameliorates Dextran Sulfate Sodium induced inflammatory bowel disease through GBP5-NLRP3 pathway.
Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef.
Interferon-Inducible Guanylate-Binding Protein 5 Inhibits Replication of Multiple Viruses by Binding to the Oligosaccharyltransferase Complex and Inhibiting Glycoprotein Maturation.
The XPO1 inhibitor selinexor ameliorates bleomycin-induced pulmonary fibrosis in mice via GBP5/NLRP3 inflammasome signaling.
The IRF1/GBP5 axis promotes osteoarthritis progression by activating chondrocyte pyroptosis.
Guanylate-binding protein 5-mediated cell-autonomous immunity suppresses inflammation in dental pulpitis: An in vitro study.
B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer.
GBP5 Identifies Immuno-Hot Tumors and Predicts the Therapeutic Response to Immunotherapy in NSCLC.
A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage.
GBP5 Inhibition Ameliorates the Progression of Lupus Nephritis by Suppressing NLRP3 Inflammasome Activation.
Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage.
PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer.
Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage.
Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.
Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ.
Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity.
Macrophages Demonstrate Guanylate-Binding Protein-Dependent and Bacterial Strain-Dependent Responses to Francisella tularensis.
Synergy of nanodiamond-doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells.
Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma.
Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.
Macrophages mediated delivery of chlorin e6 and treatment of lung cancer by photodynamic reprogramming.
cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages.
GBP5 drives malignancy of glioblastoma via the Src/ERK1/2/MMP3 pathway.
Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma.
Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System.
Cross-talk between SUMOylation and ISGylation in response to interferon.
IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.
Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion.
Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.
Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection.
miR-181a-5p Regulates TNF-α and miR-21a-5p Influences Gualynate-Binding Protein 5 and IL-10 Expression in Macrophages Affecting Host Control of Brucella abortus Infection.
IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.
LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation.
Expression of PD-L1, indoleamine 2,3-dioxygenase and the immune microenvironment in gastric adenocarcinoma.
NADPH Oxidase and Guanylate Binding Protein 5 Restrict Survival of Avirulent Type III Strains of Toxoplasma gondii in Naive Macrophages.
IFN-γ extends the immune functions of Guanylate Binding Proteins to inflammasome-independent antibacterial activities during Francisella novicida infection.
Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.
Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment.
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.
Medullary carcinoma of the colon: a distinct morphology reveals a distinctive immunoregulatory microenvironment.
Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
Che N, Zhang Y, Zhang S, Kong X, Zhang Y, Wang S, Yuan Z, Liao Y
International journal of medical sciences 2024;21(8):1385-1398
International journal of medical sciences 2024;21(8):1385-1398
Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef.
Prelli Bozzo C, Laliberté A, De Luna A, Pastorio C, Regensburger K, Krebs S, Graf A, Blum H, Volcic M, Sparrer KMJ, Kirchhoff F
Nature communications 2024 May 7;15(1):3813
Nature communications 2024 May 7;15(1):3813
Interferon-Inducible Guanylate-Binding Protein 5 Inhibits Replication of Multiple Viruses by Binding to the Oligosaccharyltransferase Complex and Inhibiting Glycoprotein Maturation.
Wang S, Li W, Wang L, Tiwari SK, Bray W, Wu L, Li N, Hui H, Clark AE, Zhang Q, Zhang L, Carlin AF, Rana TM
bioRxiv : the preprint server for biology 2024 May 3;
bioRxiv : the preprint server for biology 2024 May 3;
The XPO1 inhibitor selinexor ameliorates bleomycin-induced pulmonary fibrosis in mice via GBP5/NLRP3 inflammasome signaling.
Zhang J, Zhang Y, Chen Q, Qi Y, Zhang X
International immunopharmacology 2024 Mar 30;130:111734
International immunopharmacology 2024 Mar 30;130:111734
The IRF1/GBP5 axis promotes osteoarthritis progression by activating chondrocyte pyroptosis.
Tang H, Gong X, Dai J, Gu J, Dong Z, Xu Y, Hu Z, Zhao C, Deng J, Dong S
Journal of orthopaedic translation 2024 Jan;44:47-59
Journal of orthopaedic translation 2024 Jan;44:47-59
Guanylate-binding protein 5-mediated cell-autonomous immunity suppresses inflammation in dental pulpitis: An in vitro study.
Huang M, Zhan C, Yang B, Lu Y, Yang X, Hou J
International endodontic journal 2024 Feb;57(2):208-218
International endodontic journal 2024 Feb;57(2):208-218
B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer.
Hui YJ, Yu TT, Li LG, Peng XC, Di MJ, Liu H, Gu WL, Li TF, Zhao KL, Wang WX
Scientific reports 2024 Apr 2;14(1):7733
Scientific reports 2024 Apr 2;14(1):7733
GBP5 Identifies Immuno-Hot Tumors and Predicts the Therapeutic Response to Immunotherapy in NSCLC.
Fan H, Shi Y, Wang H, Li Y, Mei J, Xu J, Liu C
International journal of general medicine 2023;16:1757-1769
International journal of general medicine 2023;16:1757-1769
A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage.
Li LG, Yang XX, Xu HZ, Yu TT, Li QR, Hu J, Peng XC, Han N, Xu X, Chen NN, Chen X, Tang JM, Li TF
Advanced healthcare materials 2023 Nov;12(28):e2301561
Advanced healthcare materials 2023 Nov;12(28):e2301561
GBP5 Inhibition Ameliorates the Progression of Lupus Nephritis by Suppressing NLRP3 Inflammasome Activation.
Liu N, Gao Y, Liu Y, Liu D
Immunological investigations 2023 Jan;52(1):52-66
Immunological investigations 2023 Jan;52(1):52-66
Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage.
Yu TT, Hu J, Li QR, Peng XC, Xu HZ, Han N, Li LG, Yang XX, Xu X, Yang ZY, Chen H, Chen X, Wang MF, Li TF
International immunopharmacology 2023 Feb;115:109661
International immunopharmacology 2023 Feb;115:109661
PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer.
Wang C, Xu YH, Xu HZ, Li K, Zhang Q, Shi L, Zhao L, Chen X
Journal of nanobiotechnology 2023 Dec 11;21(1):476
Journal of nanobiotechnology 2023 Dec 11;21(1):476
Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage.
Li LG, Peng XC, Yu TT, Xu HZ, Han N, Yang XX, Li QR, Hu J, Liu B, Yang ZY, Xu X, Chen X, Wang MF, Li TF
Frontiers in pharmacology 2022;13:949835
Frontiers in pharmacology 2022;13:949835
Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.
Yu TT, Han N, Li LG, Peng XC, Li QR, Xu HZ, Wang XY, Yang ZY, Chen X, Wang MF, Li TF
Frontiers in pharmacology 2022;13:837784
Frontiers in pharmacology 2022;13:837784
Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ.
Coleman CS, Stanley BA, Lang CH
Inflammation 2022 Jun;45(3):1313-1331
Inflammation 2022 Jun;45(3):1313-1331
Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity.
Chowdhury D, Gardner JC, Satpati A, Nookala S, Mukundan S, Porollo A, Landero Figueroa JA, Subramanian Vignesh K
Frontiers in immunology 2021;12:755961
Frontiers in immunology 2021;12:755961
Macrophages Demonstrate Guanylate-Binding Protein-Dependent and Bacterial Strain-Dependent Responses to Francisella tularensis.
Mohammadi N, Lindgren H, Yamamoto M, Martin A, Henry T, Sjöstedt A
Frontiers in cellular and infection microbiology 2021;11:784101
Frontiers in cellular and infection microbiology 2021;11:784101
Synergy of nanodiamond-doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells.
Xu HZ, Li TF, Wang C, Ma Y, Liu Y, Zheng MY, Liu ZJ, Chen JB, Li K, Sun SK, Komatsu N, Xu YH, Zhao L, Chen X
Journal of nanobiotechnology 2021 Sep 6;19(1):268
Journal of nanobiotechnology 2021 Sep 6;19(1):268
Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma.
Li TF, Xu HZ, Xu YH, Yu TT, Tang JM, Li K, Wang C, Peng XC, Li QR, Sang XY, Zheng MY, Liu Y, Zhao L, Chen X
Molecular pharmaceutics 2021 Sep 6;18(9):3601-3615
Molecular pharmaceutics 2021 Sep 6;18(9):3601-3615
Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.
Tana FL, Guimarães ES, Cerqueira DM, Campos PC, Gomes MTR, Marinho FV, Oliveira SC
Cellular microbiology 2021 Oct;23(10):e13375
Cellular microbiology 2021 Oct;23(10):e13375
Macrophages mediated delivery of chlorin e6 and treatment of lung cancer by photodynamic reprogramming.
Yu TT, Sang XY, Han N, Peng XC, Li QR, Xu X, Xiao RC, Xu HZ, Chen X, Wang MF, Li TF
International immunopharmacology 2021 Nov;100:108164
International immunopharmacology 2021 Nov;100:108164
cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages.
Yu P, Miao Z, Li Y, Bansal R, Peppelenbosch MP, Pan Q
Gut microbes 2021 Jan-Dec;13(1):1959839
Gut microbes 2021 Jan-Dec;13(1):1959839
GBP5 drives malignancy of glioblastoma via the Src/ERK1/2/MMP3 pathway.
Yu X, Jin J, Zheng Y, Zhu H, Xu H, Ma J, Lan Q, Zhuang Z, Chen CC, Li M
Cell death & disease 2021 Feb 19;12(2):203
Cell death & disease 2021 Feb 19;12(2):203
Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma.
Liu PF, Shu CW, Lee CH, Sie HC, Liou HH, Cheng JT, Ger LP, Chen CL, Chen CC, Chen CF
Cancers 2021 Aug 11;13(16)
Cancers 2021 Aug 11;13(16)
Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System.
Wang X, Zhu H, Lei L, Zhang Y, Tang C, Wu JX, Zhou JR, Xiao XR
Frontiers in genetics 2020;11:581789
Frontiers in genetics 2020;11:581789
Cross-talk between SUMOylation and ISGylation in response to interferon.
El-Asmi F, McManus FP, Brantis-de-Carvalho CE, Valle-Casuso JC, Thibault P, Chelbi-Alix MK
Cytokine 2020 May;129:155025
Cytokine 2020 May;129:155025
IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.
Karki R, Lee E, Sharma BR, Banoth B, Kanneganti TD
Journal of immunology (Baltimore, Md. : 1950) 2020 May 1;204(9):2514-2522
Journal of immunology (Baltimore, Md. : 1950) 2020 May 1;204(9):2514-2522
Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion.
Place DE, Briard B, Samir P, Karki R, Bhattacharya A, Guy CS, Peters JL, Frase S, Vogel P, Neale G, Yamamoto M, Kanneganti TD
PLoS pathogens 2020 Mar;16(3):e1008364
PLoS pathogens 2020 Mar;16(3):e1008364
Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.
Yuan SJ, Xu YH, Wang C, An HC, Xu HZ, Li K, Komatsu N, Zhao L, Chen X
Journal of nanobiotechnology 2019 Oct 17;17(1):110
Journal of nanobiotechnology 2019 Oct 17;17(1):110
Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection.
Briard B, Karki R, Malireddi RKS, Bhattacharya A, Place DE, Mavuluri J, Peters JL, Vogel P, Yamamoto M, Kanneganti TD
Nature microbiology 2019 Feb;4(2):316-327
Nature microbiology 2019 Feb;4(2):316-327
miR-181a-5p Regulates TNF-α and miR-21a-5p Influences Gualynate-Binding Protein 5 and IL-10 Expression in Macrophages Affecting Host Control of Brucella abortus Infection.
Corsetti PP, de Almeida LA, Gonçalves ANA, Gomes MTR, Guimarães ES, Marques JT, Oliveira SC
Frontiers in immunology 2018;9:1331
Frontiers in immunology 2018;9:1331
IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.
Karki R, Lee E, Place D, Samir P, Mavuluri J, Sharma BR, Balakrishnan A, Malireddi RKS, Geiger R, Zhu Q, Neale G, Kanneganti TD
Cell 2018 May 3;173(4):920-933.e13
Cell 2018 May 3;173(4):920-933.e13
LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation.
Santos JC, Dick MS, Lagrange B, Degrandi D, Pfeffer K, Yamamoto M, Meunier E, Pelczar P, Henry T, Broz P
The EMBO journal 2018 Mar 15;37(6)
The EMBO journal 2018 Mar 15;37(6)
Expression of PD-L1, indoleamine 2,3-dioxygenase and the immune microenvironment in gastric adenocarcinoma.
Patil PA, Blakely AM, Lombardo KA, Machan JT, Miner TJ, Wang LJ, Marwaha AS, Matoso A
Histopathology 2018 Jul;73(1):124-136
Histopathology 2018 Jul;73(1):124-136
NADPH Oxidase and Guanylate Binding Protein 5 Restrict Survival of Avirulent Type III Strains of Toxoplasma gondii in Naive Macrophages.
Matta SK, Patten K, Wang Q, Kim BH, MacMicking JD, Sibley LD
mBio 2018 Aug 28;9(4)
mBio 2018 Aug 28;9(4)
IFN-γ extends the immune functions of Guanylate Binding Proteins to inflammasome-independent antibacterial activities during Francisella novicida infection.
Wallet P, Benaoudia S, Mosnier A, Lagrange B, Martin A, Lindgren H, Golovliov I, Michal F, Basso P, Djebali S, Provost A, Allatif O, Meunier E, Broz P, Yamamoto M, Py BF, Faudry E, Sjöstedt A, Henry T
PLoS pathogens 2017 Oct;13(10):e1006630
PLoS pathogens 2017 Oct;13(10):e1006630
Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.
Li P, Jiang W, Yu Q, Liu W, Zhou P, Li J, Xu J, Xu B, Wang F, Shao F
Nature 2017 Nov 16;551(7680):378-383
Nature 2017 Nov 16;551(7680):378-383
Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment.
Li TF, Li K, Wang C, Liu X, Wen Y, Xu YH, Zhang Q, Zhao QY, Shao M, Li YZ, Han M, Komatsu N, Zhao L, Chen X
Journal of controlled release : official journal of the Controlled Release Society 2017 Dec 28;268:128-146
Journal of controlled release : official journal of the Controlled Release Society 2017 Dec 28;268:128-146
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.
Man SM, Karki R, Sasai M, Place DE, Kesavardhana S, Temirov J, Frase S, Zhu Q, Malireddi RKS, Kuriakose T, Peters JL, Neale G, Brown SA, Yamamoto M, Kanneganti TD
Cell 2016 Oct 6;167(2):382-396.e17
Cell 2016 Oct 6;167(2):382-396.e17
Medullary carcinoma of the colon: a distinct morphology reveals a distinctive immunoregulatory microenvironment.
Friedman K, Brodsky AS, Lu S, Wood S, Gill AJ, Lombardo K, Yang D, Resnick MB
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 May;29(5):528-41
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 May;29(5):528-41
Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
Meunier E, Wallet P, Dreier RF, Costanzo S, Anton L, Rühl S, Dussurgey S, Dick MS, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P
Nature immunology 2015 May;16(5):476-484
Nature immunology 2015 May;16(5):476-484
The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.
Man SM, Karki R, Malireddi RK, Neale G, Vogel P, Yamamoto M, Lamkanfi M, Kanneganti TD
Nature immunology 2015 May;16(5):467-75
Nature immunology 2015 May;16(5):467-75
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
Meunier E, Dick MS, Dreier RF, Schürmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
Nature 2014 May 15;509(7500):366-70
Nature 2014 May 15;509(7500):366-70
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- U-937 cells were subjected to SDS PAGE followed by western blot with 13220-1-AP( GBP5 Antibody) at dilution of 1:1500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody 13220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue