13220-1-AP

antibody from Proteintech Group

Targeting: GBP5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

13220-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13220-1-AP, RRID:AB_2109348

Product name

GBP5 antibody

Antibody type

Polyclonal

Description

KD/KO validated GBP5 antibody (Cat. #13220-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IP, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity.

Chowdhury D, Gardner JC, Satpati A, Nookala S, Mukundan S, Porollo A, Landero Figueroa JA, Subramanian Vignesh K
Frontiers in immunology 2021;12:755961

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Synergy of nanodiamond-doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells.

Xu HZ, Li TF, Wang C, Ma Y, Liu Y, Zheng MY, Liu ZJ, Chen JB, Li K, Sun SK, Komatsu N, Xu YH, Zhao L, Chen X
Journal of nanobiotechnology 2021 Sep 6;19(1):268

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Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma.

Li TF, Xu HZ, Xu YH, Yu TT, Tang JM, Li K, Wang C, Peng XC, Li QR, Sang XY, Zheng MY, Liu Y, Zhao L, Chen X
Molecular pharmaceutics 2021 Sep 6;18(9):3601-3615

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Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.

Tana FL, Guimarães ES, Cerqueira DM, Campos PC, Gomes MTR, Marinho FV, Oliveira SC
Cellular microbiology 2021 Oct;23(10):e13375

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Macrophages mediated delivery of chlorin e6 and treatment of lung cancer by photodynamic reprogramming.

Yu TT, Sang XY, Han N, Peng XC, Li QR, Xu X, Xiao RC, Xu HZ, Chen X, Wang MF, Li TF
International immunopharmacology 2021 Nov;100:108164

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cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages.

Yu P, Miao Z, Li Y, Bansal R, Peppelenbosch MP, Pan Q
Gut microbes 2021 Jan-Dec;13(1):1959839

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GBP5 drives malignancy of glioblastoma via the Src/ERK1/2/MMP3 pathway.

Yu X, Jin J, Zheng Y, Zhu H, Xu H, Ma J, Lan Q, Zhuang Z, Chen CC, Li M
Cell death & disease 2021 Feb 19;12(2):203

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Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma.

Liu PF, Shu CW, Lee CH, Sie HC, Liou HH, Cheng JT, Ger LP, Chen CL, Chen CC, Chen CF
Cancers 2021 Aug 11;13(16)

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Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System.

Wang X, Zhu H, Lei L, Zhang Y, Tang C, Wu JX, Zhou JR, Xiao XR
Frontiers in genetics 2020;11:581789

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Cross-talk between SUMOylation and ISGylation in response to interferon.

El-Asmi F, McManus FP, Brantis-de-Carvalho CE, Valle-Casuso JC, Thibault P, Chelbi-Alix MK
Cytokine 2020 May;129:155025

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IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.

Karki R, Lee E, Sharma BR, Banoth B, Kanneganti TD
Journal of immunology (Baltimore, Md. : 1950) 2020 May 1;204(9):2514-2522

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Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion.

Place DE, Briard B, Samir P, Karki R, Bhattacharya A, Guy CS, Peters JL, Frase S, Vogel P, Neale G, Yamamoto M, Kanneganti TD
PLoS pathogens 2020 Mar;16(3):e1008364

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Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.

Yuan SJ, Xu YH, Wang C, An HC, Xu HZ, Li K, Komatsu N, Zhao L, Chen X
Journal of nanobiotechnology 2019 Oct 17;17(1):110

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Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection.

Briard B, Karki R, Malireddi RKS, Bhattacharya A, Place DE, Mavuluri J, Peters JL, Vogel P, Yamamoto M, Kanneganti TD
Nature microbiology 2019 Feb;4(2):316-327

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miR-181a-5p Regulates TNF-α and miR-21a-5p Influences Gualynate-Binding Protein 5 and IL-10 Expression in Macrophages Affecting Host Control of Brucella abortus Infection.

Corsetti PP, de Almeida LA, Gonçalves ANA, Gomes MTR, Guimarães ES, Marques JT, Oliveira SC
Frontiers in immunology 2018;9:1331

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IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.

Karki R, Lee E, Place D, Samir P, Mavuluri J, Sharma BR, Balakrishnan A, Malireddi RKS, Geiger R, Zhu Q, Neale G, Kanneganti TD
Cell 2018 May 3;173(4):920-933.e13

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LPS targets host guanylate-binding proteins to the bacterial outer membrane for non-canonical inflammasome activation.

Santos JC, Dick MS, Lagrange B, Degrandi D, Pfeffer K, Yamamoto M, Meunier E, Pelczar P, Henry T, Broz P
The EMBO journal 2018 Mar 15;37(6)

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Expression of PD-L1, indoleamine 2,3-dioxygenase and the immune microenvironment in gastric adenocarcinoma.

Patil PA, Blakely AM, Lombardo KA, Machan JT, Miner TJ, Wang LJ, Marwaha AS, Matoso A
Histopathology 2018 Jul;73(1):124-136

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NADPH Oxidase and Guanylate Binding Protein 5 Restrict Survival of Avirulent Type III Strains of Toxoplasma gondii in Naive Macrophages.

Matta SK, Patten K, Wang Q, Kim BH, MacMicking JD, Sibley LD
mBio 2018 Aug 28;9(4)

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IFN-γ extends the immune functions of Guanylate Binding Proteins to inflammasome-independent antibacterial activities during Francisella novicida infection.

Wallet P, Benaoudia S, Mosnier A, Lagrange B, Martin A, Lindgren H, Golovliov I, Michal F, Basso P, Djebali S, Provost A, Allatif O, Meunier E, Broz P, Yamamoto M, Py BF, Faudry E, Sjöstedt A, Henry T
PLoS pathogens 2017 Oct;13(10):e1006630

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Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.

Li P, Jiang W, Yu Q, Liu W, Zhou P, Li J, Xu J, Xu B, Wang F, Shao F
Nature 2017 Nov 16;551(7680):378-383

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Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment.

Li TF, Li K, Wang C, Liu X, Wen Y, Xu YH, Zhang Q, Zhao QY, Shao M, Li YZ, Han M, Komatsu N, Zhao L, Chen X
Journal of controlled release : official journal of the Controlled Release Society 2017 Dec 28;268:128-146

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IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.

Man SM, Karki R, Sasai M, Place DE, Kesavardhana S, Temirov J, Frase S, Zhu Q, Malireddi RKS, Kuriakose T, Peters JL, Neale G, Brown SA, Yamamoto M, Kanneganti TD
Cell 2016 Oct 6;167(2):382-396.e17

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Medullary carcinoma of the colon: a distinct morphology reveals a distinctive immunoregulatory microenvironment.

Friedman K, Brodsky AS, Lu S, Wood S, Gill AJ, Lombardo K, Yang D, Resnick MB
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 May;29(5):528-41

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Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.

Meunier E, Wallet P, Dreier RF, Costanzo S, Anton L, Rühl S, Dussurgey S, Dick MS, Kistner A, Rigard M, Degrandi D, Pfeffer K, Yamamoto M, Henry T, Broz P
Nature immunology 2015 May;16(5):476-484

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The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.

Man SM, Karki R, Malireddi RK, Neale G, Vogel P, Yamamoto M, Lamkanfi M, Kanneganti TD
Nature immunology 2015 May;16(5):467-75

---

Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.

Meunier E, Dick MS, Dreier RF, Schürmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
Nature 2014 May 15;509(7500):366-70

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

U-937 cells were subjected to SDS PAGE followed by western blot with 13220-1-AP( GBP5 Antibody) at dilution of 1:1500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody 13220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 13220-1-AP(GBP5 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue