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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 517202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#517202, RRID:AB_10690819
- Product name
- Purified anti-human IL-33
- Antibody type
- Monoclonal
- Antigen
- Mature recombinant protein human Interleukin-33 (Ser112-Thr270)
- Description
- IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C.
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Supportive validation
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- Experimental details
- 500 ng (Lane 1), 200 ng (Lane 2), and 50 ng (Lane 3) of human IL-33 (Ser112-Thr270) recombinant proteins were separated by electrophoresis, transferred to nitrocellulose, and probed with monoclonal hIL-33 antibody (clone BL35172). Protein were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
