Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB200-353 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-353, RRID:AB_2092161
- Product name
- Rabbit Polyclonal DHX36 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The DEAH-box helicase DHX36 mediates dendritic localization of the neuronal precursor-microRNA-134.
Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production.
Aspartate-glutamate-alanine-histidine box motif (DEAH)/RNA helicase A helicases sense microbial DNA in human plasmacytoid dendritic cells.
Bicker S, Khudayberdiev S, Weiß K, Zocher K, Baumeister S, Schratt G
Genes & development 2013 May 1;27(9):991-6
Genes & development 2013 May 1;27(9):991-6
Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production.
Naji S, Ambrus G, Cimermančič P, Reyes JR, Johnson JR, Filbrandt R, Huber MD, Vesely P, Krogan NJ, Yates JR 3rd, Saphire AC, Gerace L
Molecular & cellular proteomics : MCP 2012 Apr;11(4):M111.015313
Molecular & cellular proteomics : MCP 2012 Apr;11(4):M111.015313
Aspartate-glutamate-alanine-histidine box motif (DEAH)/RNA helicase A helicases sense microbial DNA in human plasmacytoid dendritic cells.
Kim T, Pazhoor S, Bao M, Zhang Z, Hanabuchi S, Facchinetti V, Bover L, Plumas J, Chaperot L, Qin J, Liu YJ
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 24;107(34):15181-6
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 24;107(34):15181-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX36 Antibody [NB200-353] - Detection of Human DHX36 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug) and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody NB200-353 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX36 Antibody [NB200-353] - Detection of DHX36 on HeLa whole cell lystate using NB200-353.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX36 Antibody [NB200-353] - Whole cell lysate from NIH3T3 (50ug), TCKM (50ug) and Renca (50ug) cells. Antibodies: Affinity purified rabbit antiDHX36 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DHX36 Antibody [NB200-353] - Detection of human DHX36 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody NB200-353 (lot NB200-353-2) used for IP at 6 ug/mg lysate. DHX36 was also immunoprecipitated by a previous lot (NB200-353-1) of this antibody. For blotting immunoprecipitated DHX36, NB200-353 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DHX36 Antibody [NB200-353] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DHX36 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB