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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00055777-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055777-M01, RRID:AB_2139880
- Product name
- Mouse Monoclonal MBD5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MBD5 - methyl-CpG binding domain protein 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of monoclonal antibody to MBD5 on HeLa cell . Antibody concentration 10 ug/ml.
