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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [3]
- ELISA [1]
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- Product number
- H00009197-M07 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009197-M07, RRID:AB_2189943
- Product name
- Mouse Monoclonal Acetyl-coenzyme A transporter 1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SLC33A1 (3A4)
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SLC33A1/AT-1 protein regulates the induction of autophagy downstream of IRE1/XBP1 pathway.
AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.
Pehar M, Jonas MC, Hare TM, Puglielli L
The Journal of biological chemistry 2012 Aug 24;287(35):29921-30
The Journal of biological chemistry 2012 Aug 24;287(35):29921-30
AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.
Jonas MC, Pehar M, Puglielli L
Journal of cell science 2010 Oct 1;123(Pt 19):3378-88
Journal of cell science 2010 Oct 1;123(Pt 19):3378-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Acetyl-coenzyme A transporter 1 Antibody (3A4) [H00009197-M07] - Western blot analysis of SLC33A1 over-expressed 293 cell line, cotransfected with SLC33A1 Validated Chimera RNAi or non-transfected control. Blot probed with H00009197-M07. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Acetyl-coenzyme A transporter 1 Antibody (3A4) [H00009197-M07] - Analysis of SLC33A1 expression in transfected 293T cell line by SLC33A1 monoclonal antibody (M07), clone 3A4. Lane 1: SLC33A1 transfected lysatE (60.9 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Acetyl-coenzyme A transporter 1 Antibody (3A4) [H00009197-M07] - Analysis of SLC33A1 expression in PC-12 (Cat # L012V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Acetyl-coenzyme A transporter 1 Antibody (3A4) [H00009197-M07] - Detection limit for recombinant GST tagged SLC33A1 is 0.03 ng/ml as a capture antibody.
