NBP1-97622

antibody from Novus Biologicals

Targeting: TNFSF13B BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP1-97622

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-97622, RRID:AB_11189480

Product name

Rat Monoclonal BAFF/BLyS/TNFSF13B Antibody

Antibody type

Monoclonal

Description

Protein G purified. Recognizes membrane-bound and soluble human and mouse (weak) BAFF.

Reactivity

Human

Host

Rat

Isotype

IgM

Vial size

0.05 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Expression of TNF-superfamily members BAFF and APRIL in breast cancer: immunohistochemical study in 52 invasive ductal breast carcinomas.

Pelekanou V, Kampa M, Kafousi M, Darivianaki K, Sanidas E, Tsiftsis DD, Stathopoulos EN, Tsapis A, Castanas E
BMC cancer 2008 Mar 20;8:76

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In vitro and in vivo activation induces BAFF and APRIL expression in B cells.

Chu VT, Enghard P, Riemekasten G, Berek C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 1;179(9):5947-57

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In vitro and in vivo activation induces BAFF and APRIL expression in B cells.

Chu VT, Enghard P, Riemekasten G, Berek C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 1;179(9):5947-57

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Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.

Ogden CA, Pound JD, Batth BK, Owens S, Johannessen I, Wood K, Gregory CD
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23

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Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.

Ogden CA, Pound JD, Batth BK, Owens S, Johannessen I, Wood K, Gregory CD
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23

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Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation with disease activity and patient outcome.

Novak AJ, Grote DM, Stenson M, Ziesmer SC, Witzig TE, Habermann TM, Harder B, Ristow KM, Bram RJ, Jelinek DF, Gross JA, Ansell SM
Blood 2004 Oct 15;104(8):2247-53

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BAFF production by antigen-presenting cells provides T cell co-stimulation.

Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, Roosnek E, Tschopp J, Schneider P, French LE
International immunology 2004 Mar;16(3):467-75

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BAFF production by antigen-presenting cells provides T cell co-stimulation.

Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, Roosnek E, Tschopp J, Schneider P, French LE
International immunology 2004 Mar;16(3):467-75

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Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL.

He B, Chadburn A, Jou E, Schattner EJ, Knowles DM, Cerutti A
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):3268-79

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Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL.

He B, Chadburn A, Jou E, Schattner EJ, Knowles DM, Cerutti A
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):3268-79

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Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival.

Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF
Blood 2004 Jan 15;103(2):689-94

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Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjögren's syndrome.

Groom J, Kalled SL, Cutler AH, Olson C, Woodcock SA, Schneider P, Tschopp J, Cachero TG, Batten M, Wheway J, Mauri D, Cavill D, Gordon TP, Mackay CR, Mackay F
The Journal of clinical investigation 2002 Jan;109(1):59-68

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.