H00010673-M04

antibody from Abnova Corporation

Targeting: TNFSF13B BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010673-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010673-M04, RRID:AB_10628592

Product name

TNFSF13B monoclonal antibody (M04), clone 2C11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant TNFSF13B.

Antigen sequence

MDDSTEREQSRLTSCLKKREEMKLKECVSILPRKE
SPSVRSSKDGKLLAATLLLALLSCCLTVVSFYQVA
ALQGDLASLRAELQGHHAEKLPAGAGAPKAGLEEA
PAVTAGLKIFEPPAPGEGNSSQNSRNKRAVQGPEE
TVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRG
SALEEKENKILVKETGYFFIYGQVLYTDKTYAMGH
LIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSC
YSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFG
ALKLL


Isotype

IgG

Antibody clone number

2C11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TNFSF13B expression in transfected 293T cell line by TNFSF13B monoclonal antibody (M04), clone 2C11.Lane 1: TNFSF13B transfected lysate(31.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TNFSF13B is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of TNFSF13B transfected lysate using anti-TNFSF13B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TNFSF13B MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol