17-9017-41
antibody from Invitrogen Antibodies
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
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Validation data
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- Product number
- 17-9017-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD257 (BAFF, BLyS) Monoclonal Antibody (1D6), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1 (TNF and apoptosis leukocyte-expressed ligand-1), THANK (TNF homologue that activates apoptosis, nuclear factor KappaB and c-Jun NH2-terminal kinase) and zTNF4. BLyS, a member of the TNF family, is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes lineage is regulated by IFN gamma. BLyS acts as a potent B cell growth factor and costimulator of Ig production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease characterized by the presence of autoantibodies against nuclear antigens and immune complexes deposited in the kidney. Data from animal models imply a role for BLyS in human autoimmune diseases. To date BCMA, TACI and BR3 have been identified as receptors for BLyS. APRIL, another TNF family member, and BLyS can form active heterotrimeric molecules when coexpressed and these circulating heterotrimers are present in serum samples from patients with systemic immune-based rheumatic diseases. Furthermore, it has been reported that BLyS and APRIL, share BCMA and TACI as their receptors.
- Antibody clone number
- 1D6
- Concentration
- 5 µL/Test
Submitted references Positive and negative cooperativity of TNF and Interferon-γ in regulating synovial fibroblast function and B cell survival in fibroblast/B cell co-cultures.
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex.
BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2.
BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases.
Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators.
Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency.
Lowin T, Anssar TM, Bäuml M, Classen T, Schneider M, Pongratz G
Scientific reports 2020 Jan 21;10(1):780
Scientific reports 2020 Jan 21;10(1):780
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
Bornacelly A, Mercado D, Acevedo N, Caraballo L
BMC immunology 2014 Jun 7;15:22
BMC immunology 2014 Jun 7;15:22
BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex.
Hase H, Kanno Y, Kojima M, Hasegawa K, Sakurai D, Kojima H, Tsuchiya N, Tokunaga K, Masawa N, Azuma M, Okumura K, Kobata T
Blood 2004 Mar 15;103(6):2257-65
Blood 2004 Mar 15;103(6):2257-65
BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2.
Kayagaki N, Yan M, Seshasayee D, Wang H, Lee W, French DM, Grewal IS, Cochran AG, Gordon NC, Yin J, Starovasnik MA, Dixit VM
Immunity 2002 Oct;17(4):515-24
Immunity 2002 Oct;17(4):515-24
BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases.
Roschke V, Sosnovtseva S, Ward CD, Hong JS, Smith R, Albert V, Stohl W, Baker KP, Ullrich S, Nardelli B, Hilbert DM, Migone TS
Journal of immunology (Baltimore, Md. : 1950) 2002 Oct 15;169(8):4314-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Oct 15;169(8):4314-21
Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators.
Hsu BL, Harless SM, Lindsley RC, Hilbert DM, Cancro MP
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):5993-6
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):5993-6
Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency.
Yan M, Brady JR, Chan B, Lee WP, Hsu B, Harless S, Cancro M, Grewal IS, Dixit VM
Current biology : CB 2001 Oct 2;11(19):1547-52
Current biology : CB 2001 Oct 2;11(19):1547-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human BLyS-transfected cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD257 (BAFF, BLyS) APC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Cell surface levels of membrane-bound BAFF ( A ), CD40L ( B ), IFN-gammaR ( C ), ObR ( D ), Fn14 ( E ), TACI ( F ), BAFF-R ( G ) and BCMA ( H ) after stimulation with given cytokines for 72 h. The upper panels depict exemplary histograms after flow cytometric analyses. All FACS measurements were normalized to the geometric mean of the unstimulated control value, which was set at 100% (dotted line). P-values are given in the graph. The general linear model with Bonferroni correction was used for all comparisons versus control.