ALX-804-128-C100

antibody from Enzo Life Sciences

Targeting: TNFSF13B BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-804-128-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-128-C100, RRID:AB_2050782

Product name

BAFF (human) monoclonal antibody (Buffy-1)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

Buffy-1

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone.

Moreaux J, Legouffe E, Jourdan E, Quittet P, Rème T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K
Blood 2004 Apr 15;103(8):3148-57

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Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival.

Novak AJ, Bram RJ, Kay NE, Jelinek DF
Blood 2002 Oct 15;100(8):2973-9

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BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population.

Thompson JS, Schneider P, Kalled SL, Wang L, Lefevre EA, Cachero TG, MacKay F, Bixler SA, Zafari M, Liu ZY, Woodcock SA, Qian F, Batten M, Madry C, Richard Y, Benjamin CD, Browning JL, Tsapis A, Tschopp J, Ambrose C
The Journal of experimental medicine 2000 Jul 3;192(1):129-35

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A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth.

Rennert P, Schneider P, Cachero TG, Thompson J, Trabach L, Hertig S, Holler N, Qian F, Mullen C, Strauch K, Browning JL, Ambrose C, Tschopp J
The Journal of experimental medicine 2000 Dec 4;192(11):1677-84

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BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth.

Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, Bixler S, Acha-Orbea H, Valmori D, Romero P, Werner-Favre C, Zubler RH, Browning JL, Tschopp J
The Journal of experimental medicine 1999 Jun 7;189(11):1747-56

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of BAFF (human), mAb (Buffy-1) (Prod. No. ALX-804-128): Lane 1: MW marker; Lane 2: BAFF (soluble) (human), (recombinant) (Prod. No. ALX-522-025).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Membrane-bound human BAFF detected byÊMAb to BAFF (human) (Buffy-1) (Prod. No. ALX-804-128). Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml. Method: 293T Cells (5 x 10E5) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4µg/ml of MAb to BAFF (human) (Buffy-1). After washing in FACS buffer, FITC-conjugated antibody to rat IgG(ALX-211-051) was added and cells were analyzed by flow cytometry.