ALX-804-128-C100
antibody from Enzo Life Sciences
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [3]
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- Product number
- ALX-804-128-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-128-C100, RRID:AB_2050782
- Product name
- BAFF (human) monoclonal antibody (Buffy-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- Buffy-1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone.
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival.
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population.
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth.
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth.
Moreaux J, Legouffe E, Jourdan E, Quittet P, Rème T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K
Blood 2004 Apr 15;103(8):3148-57
Blood 2004 Apr 15;103(8):3148-57
Aberrant expression of B-lymphocyte stimulator by B chronic lymphocytic leukemia cells: a mechanism for survival.
Novak AJ, Bram RJ, Kay NE, Jelinek DF
Blood 2002 Oct 15;100(8):2973-9
Blood 2002 Oct 15;100(8):2973-9
BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population.
Thompson JS, Schneider P, Kalled SL, Wang L, Lefevre EA, Cachero TG, MacKay F, Bixler SA, Zafari M, Liu ZY, Woodcock SA, Qian F, Batten M, Madry C, Richard Y, Benjamin CD, Browning JL, Tsapis A, Tschopp J, Ambrose C
The Journal of experimental medicine 2000 Jul 3;192(1):129-35
The Journal of experimental medicine 2000 Jul 3;192(1):129-35
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth.
Rennert P, Schneider P, Cachero TG, Thompson J, Trabach L, Hertig S, Holler N, Qian F, Mullen C, Strauch K, Browning JL, Ambrose C, Tschopp J
The Journal of experimental medicine 2000 Dec 4;192(11):1677-84
The Journal of experimental medicine 2000 Dec 4;192(11):1677-84
BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth.
Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, Bixler S, Acha-Orbea H, Valmori D, Romero P, Werner-Favre C, Zubler RH, Browning JL, Tschopp J
The Journal of experimental medicine 1999 Jun 7;189(11):1747-56
The Journal of experimental medicine 1999 Jun 7;189(11):1747-56
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of BAFF (human), mAb (Buffy-1) (Prod. No. ALX-804-128): Lane 1: MW marker; Lane 2: BAFF (soluble) (human), (recombinant) (Prod. No. ALX-522-025).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Membrane-bound human BAFF detected byÊMAb to BAFF (human) (Buffy-1) (Prod. No. ALX-804-128). Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml. Method: 293T Cells (5 x 10E5) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 4µg/ml of MAb to BAFF (human) (Buffy-1). After washing in FACS buffer, FITC-conjugated antibody to rat IgG(ALX-211-051) was added and cells were analyzed by flow cytometry.