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LearnALX-804-131-C100
antibody from Enzo Life Sciences
Targeting: TNFSF13B
BAFF, BLYS, CD257, TALL-1, TALL1, THANK, TNFSF20
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ALX-804-131-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-131-C100, RRID:AB_2050786
- Product name
- BAFF monoclonal antibody (Buffy-2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Purified by IgM purification HP column.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgM
- Antibody clone number
- Buffy-2
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Expression of TNF-superfamily members BAFF and APRIL in breast cancer: immunohistochemical study in 52 invasive ductal breast carcinomas.
In vitro and in vivo activation induces BAFF and APRIL expression in B cells.
Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjögren's syndrome.
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation with disease activity and patient outcome.
BAFF production by antigen-presenting cells provides T cell co-stimulation.
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL.
Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival.
Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjögren's syndrome.
Pelekanou V, Kampa M, Kafousi M, Darivianaki K, Sanidas E, Tsiftsis DD, Stathopoulos EN, Tsapis A, Castanas E
BMC cancer 2008 Mar 20;8:76
BMC cancer 2008 Mar 20;8:76
In vitro and in vivo activation induces BAFF and APRIL expression in B cells.
Chu VT, Enghard P, Riemekasten G, Berek C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 1;179(9):5947-57
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 1;179(9):5947-57
Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjögren's syndrome.
Jonsson MV, Szodoray P, Jellestad S, Jonsson R, Skarstein K
Journal of clinical immunology 2005 May;25(3):189-201
Journal of clinical immunology 2005 May;25(3):189-201
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
Ogden CA, Pound JD, Batth BK, Owens S, Johannessen I, Wood K, Gregory CD
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23
Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation with disease activity and patient outcome.
Novak AJ, Grote DM, Stenson M, Ziesmer SC, Witzig TE, Habermann TM, Harder B, Ristow KM, Bram RJ, Jelinek DF, Gross JA, Ansell SM
Blood 2004 Oct 15;104(8):2247-53
Blood 2004 Oct 15;104(8):2247-53
BAFF production by antigen-presenting cells provides T cell co-stimulation.
Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, Roosnek E, Tschopp J, Schneider P, French LE
International immunology 2004 Mar;16(3):467-75
International immunology 2004 Mar;16(3):467-75
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL.
He B, Chadburn A, Jou E, Schattner EJ, Knowles DM, Cerutti A
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):3268-79
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):3268-79
Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival.
Novak AJ, Darce JR, Arendt BK, Harder B, Henderson K, Kindsvogel W, Gross JA, Greipp PR, Jelinek DF
Blood 2004 Jan 15;103(2):689-94
Blood 2004 Jan 15;103(2):689-94
Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjögren's syndrome.
Groom J, Kalled SL, Cutler AH, Olson C, Woodcock SA, Schneider P, Tschopp J, Cachero TG, Batten M, Wheway J, Mauri D, Cavill D, Gordon TP, Mackay CR, Mackay F
The Journal of clinical investigation 2002 Jan;109(1):59-68
The Journal of clinical investigation 2002 Jan;109(1):59-68
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
Supportive validation
- Submitted by
- ricca.dario
- Main image
- Experimental details
- Rat anti-Human Baff/TNFSF 13B/CD257 (ALX-804-131-C100). Baff was detected in FFPE human tonsil using HIER pH6 and 20 µg/mL (1:50) BAFF mAb (Buffy-2) IgM Rat anti-Human, 1h at room temperature. Tissue was stained with 1:100 Goat anti-Rat IgM HRP (Abcam, ab97180)and counterstained with hematoxylin.
- Sample type
- Tonsil
- Primary Ab dilution
- 1:50
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:100
