Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27838 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLMN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, NIH-3T3.
- Concentration
- 0.23 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLMN using 30 µg of A) Jurkat and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GLMN polyclonal antibody (Product # PA5-27838) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GLMN was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GLMN Polyclonal Antibody (Product # PA5-27838) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GLMN Polyclonal Antibody (Product # PA5-27838). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 . 7.5% SDS PAGE. GLMN Polyclonal Antibody (Product # PA5-27838) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GLMN was performed in Jurkat cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GLMN Polyclonal Antibody (Product # PA5-27838) diluted at 1:100. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GLMN was performed in paraffin-embedded mouse duodenum tissue using GLMN Polyclonal Antibody (Product # PA5-27838) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GLMN was performed in paraffin-embedded rat stomach tissue using GLMN Polyclonal Antibody (Product # PA5-27838) at a dilution of 1:500.